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Purified Anti-Human CD45 (HI30) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-F, FC, ICC, IP |
|---|---|
| Isotype | Mouse IgG1, kappa |
| Concentration | 0.5 mg/mL |
| Reactivity | Human |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2 |
| Host | Mouse |
| Gene ID | 5788 |
|---|---|
| Gene Name | PTPRC |
| Alternative Name(s) | Leukocyte Common Antigen, LCA, Ly-5 |
| Format | Purified |
| Preparation | This monoclonal antibody preparation was purified from tissue culture supernatant via affinity chromatography. For In Vivo Ready™ (IVR) products, each preparation is also evaluated for endotoxin levels using the LAL assay. It is recommended to store the product undiluted at 4°C. Do not freeze. |
| Application Notes | This purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Citations are provided as a convenience to you - please consult Materials and Methods sections for additional details about the use of any product in these publications. |
| Storage Conditions | 2-8°C |
Strowig T, Rongvaux A, Rathinam C, Takizawa H, Borsotti C, Philbrick W, Eynon EE, Manz MG, and Flavell RA. 2011. Proc. Natl. Acad. Sci. 108: 13218-13223. (Flow Cytometry)
Kim M-H, Suh H-S, Si Q, Terman BE, and Lee SC. 2006. J. Virol. 80: 62-72. (in vitro blocking, Western Blot)
Zhang M and Varki A. 2004. Glycobiology. 14: 939-949. (Immunoprecipitation)
Gelbmann CM, Leeb SN, Vogl D, Maendel M, Herfarth H, Scholmerich J, Falk W, and Rogler G. 2003. Gut. 52:1448-1456. (Immunocytochemistry)
Yamada T, Zhu D, Saxon A, and Zhang K. 2002. J. Biol. Chem. 277(32): 28830-28835. (in vitro blocking)
Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)
Goto E, Kohrogi H, Hirata N, Tsumori K, Hirosako S, Hamamoto J, Fujii K, Kawano O, and Ando M. 2000. Am. J. Respir. Cell Mol. Biol. 22: 405. (Immunohistochemistry – frozen tissue)
Esser MT, Graham DR, Coren LV, Trubey CM, Bess JW, Arthur LO, Ott DE, and Lifson JD. 2001. J. Virol. 75(13)6173-6182. (Western Blot)
Provided below are standard protocols that you may find useful for product applications.
Background
The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.
The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.
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